Skip to main content
SearchLoginLogin or Signup

Review 2: "Development of an automated chemiluminescence assay system for quantitative measurement of multiple anti-SARS-CoV-2 antibodies"

The authors develop a new chemiluminescence assay system for measurement of SARS-CoV-2 antibodies. However, reviewers suggest that the study is potentially informative, though the lack of analytical validation in the study limits the generalizability of the findings.

Published onDec 09, 2020
Review 2: "Development of an automated chemiluminescence assay system for quantitative measurement of multiple anti-SARS-CoV-2 antibodies"
1 of 2
key-enterThis Pub is a Review of
Development of an automated chemiluminescence assay system for quantitative measurement of multiple anti-SARS-CoV-2 antibodies
Description

Objective: Serological tests for COVID-19 have been instrumental in studying the epidemiology of the disease. However, the performance of the currently available tests is plagued by the problem of variability. We have developed a high-throughput serological test capable of simultaneously detecting total immunoglobulins (Ig) and immunoglobulin G (IgG) against two of the most immunologically relevant SARS-CoV-2 antigens, nucleocapsid protein (NP) and spike protein (SP) and report its performance in detecting COVID-19 in clinical samples. Methods: We designed and prepared reagents for measuring NP-IgG, NP-Total Ig, SP-IgG, and SP-Total Ig (using N-terminally truncated NP (ΔN-NP) or receptor-binding domain (RBD) antigen) on the advanced chemiluminescence enzyme immunoassay system TOSOH AIA-CL. After determining the basal thresholds based on 17 sera obtained from confirmed COVID-19 patients and 600 negative sera. Subsequently, the clinical validity of the assay was evaluated using independent 202 positive samples and 1,000 negative samples from healthy donors. Results: All of the four test parameters showed 100% specificity individually (1,000/1,000; 95%CI, 99.63-100). The sensitivity of the assay increased proportionally to the elapsed time from symptoms onset, and all the tests achieved 100% sensitivity (153/153; 95%CI, 97.63-100) after 13 days from symptoms onset. NP-Total Ig was the earliest to attain maximal sensitivity among the other antibodies tested. Conclusion: Our newly developed serological testing exhibited 100% sensitivity and specificity after 13 days from symptoms onset. Hence, it could be used as a reliable method for accurate detection of COVID-19 patients and to evaluate seroprevalence and possibly for surrogate assessment of herd immunity.

RR:C19 Evidence Scale rating by reviewer:

  • Strong. The main study claims are very well-justified by the data and analytic methods used. There is little room for doubt that the study produced has very similar results and conclusions as compared with the hypothetical ideal study. The study’s main claims should be considered conclusive and actionable without reservation.

***************************************

Review:

The authors in this paper developed a new chemiluminescence assay system for quantitative measurement of multiple anti-SARS-CoV-2 antibodies. The developed serological testing exhibited 100% sensitivity and specificity after 13 days from the onset of symptoms. The work is clearly and accurately presented, is well-structured, and well-written. The work used a proper number of healthy donors and good statistics.

The article can be suitable for publication but needs some minor revision:
Since the test is new and developed by the authors, they should insert the accuracy data (within run and between run precision) and, if available, data of linearity.

Comments
1
Vape Factory:

http://xpresscience.com/xs/members/vapenew/

http://xpresscience.com/xs/members/vapenew/activity/556131/

https://www.qantumthemes.com/helpdesk/forums/users/vapecig/

https://blender3d.com.ua/forums/users/vapenew/

https://ramzine.co.uk/author/vapenew/

https://study.physiotutors.com/members/vapecig/

https://thecardinalnation.com/forums/users/vapecig/

https://www.hackerrank.com/ecigator

https://learn.centa.org/forums/users/vapecig/

http://mundo.boachsoft.com/forums/users/vapecig/

https://magnifiedads.com/user/vapenew

https://www.cooplink.nl/profile/vapecig

https://organicsnewsbrasil.com.br/author/vapecig/

https://l2oldskool.org/forums/users/vapenew/

https://www.themetim.com/forums/users/vapecig/

https://pairup.makers.tech/en/vapecig

https://dadstreamer.com/community/profile/vapecig/

https://southwaleschronicle.co.uk/blog/author/vapenew/

https://www.mds-foundation.org/forums/users/vapecig/

https://kampus.dictio.id/forums/users/vapecig/

https://ismschools.com.au/forums/users/vapecig/

http://f319.com/members/vapecig.804549/

https://www.pedalroom.com/members/vapecig

https://bmt31.ru/author/vapecig/

https://app.roll20.net/users/10983309/vape-f

https://www.inzercia.rimava.sk/author/vapecig/

https://community.hodinkee.com/members/vapecig

https://www.thestudentroom.co.uk/member.php?u=6007958

https://photozou.jp/user/top/3324700

http://photozou.jp/user/top/3324700

https://edshelf.com/profile/vapecig

https://dzone.com/users/4780742/vapecig.html

https://wallhaven.cc/user/vapecig

https://booklog.jp/users/vapecig/profile

https://www.nguyenlediep.biz/members/vapecig.55061/

https://alcohol.clinicalencounters.com/members/vapecig/

https://newsknol.com/author/vapecig/

https://public.tableau.com/app/profile/vapecig

https://www.theverge.com/users/vapecig

https://photho.vn/shop/vape-factory-iaax91700

http://uid.me/vape_factory

https://www.aeriagames.com/user/ecigator/

https://www.quia.com/profiles/matthew547m

https://play.eslgaming.com/player/18491387/

https://www.worldheritagesite.org/community/vapecig

https://play.eslgaming.com/player/myinfos/18491387/

https://www.craftsforum.co.uk/member/209968-vapecig/visitormessage/1131174-visitor-message-from-vapecig

https://forums.oceancity.com/member/27123-vapecig/visitormessage/65019-visitor-message-from-vapecig

https://www.naucmese.cz/matthew-ma

https://www.decentralized.com/chapters/members/ma-m/profile/

https://econjobs.nabe.com/profile/ecigator/1822457/

https://miningjobs.smenet.org/profile/ecigator/1822462/

https://vapelife.coffeecup.com/index.html

https://coderwall.com/vapecig

https://discover.events.com/profile/ecigator/3648873/

https://lemon.cs.elte.hu/trac/lemon/ticket/670

https://valetinowiki.racing/wiki/Disposable_vape

https://www.feedsfloor.com/profile/vapecig

https://www.longisland.com/profile/vapecig

https://iq.worldcrunch.com/profile/39483

https://www.sqlservercentral.com/forums/user/vapecig

https://godotengine.org/qa/user/vapecig

https://www.multichain.com/qa/user/vapecig

https://nowewyrazy.uw.edu.pl/profil/vapecig

https://www.myminifactory.com/users/vapecig

https://3dprintboard.com/member.php?94484-vapecig

http://52040.dynamicboard.de/u202_vapecig.html

http://cloudsdeal.xobor.de/u14370_vapecig.html

https://patch.com/users/matthew-ma

https://demo.mahara.org/blocktype/wall/wall.php?id=1195

https://libremedia.video/a/vapecig/video-channels

https://dnk.video/a/vapecig/video-channels

https://birdtu.be/a/vapecig/video-channels

https://tv.atmx.ca/a/vapecig/video-channels

https://tube.rhythms-of-resistance.org/a/vapecig/video-channels

https://video.telemillevaches.net/a/vapecig/video-channels

https://gegenstimme.tv/a/vapecig/video-channels

https://tubedu.org/a/vapecig/video-channels

https://goredb.com/a/vapecig/video-channels

https://video.snug.moe/a/vapecig/video-channels

https://videos.trom.tf/a/vapecig/video-channels

https://tube.chocoflan.net/a/vapecig/video-channels

https://peertube.stream/a/vapecig/video-channels

https://watch.vtopia.live/a/vapecig/video-channels

https://tube.xy-space.de/a/vapecig/video-channels

https://peertube.virtual-assembly.org/accounts/vapecig/video-channels

https://video.liberta.vip/a/vapecig/video-channels

https://peertube.we-keys.fr/a/vapecig/video-channels

https://tube.spdns.org/a/vapecig/video-channels

https://peertube.devol.it/a/vapecig/video-channels

https://player.ojamajo.moe/a/vapecig/video-channels

https://peervideo.ru/a/vapecig/video-channels

https://peertube.nz/a/vapecig/video-channels

https://skeptikon.fr/a/vapecig/video-channels

https://video.occm.cc/a/vapecig/video-channels

https://kraut.zone/a/vapecig/video-channels

https://video.hardlimit.com/a/vapecig/video-channels

https://noiz.ie/a/vapecig/video-channels

https://pewflix.com/a/vapecig/video-channels

https://tubee.fr/a/vapecig/video-channels

https://hitchtube.fr/a/vapecig/video-channels

https://tube.p2p.legal/a/vapecig/video-channels

https://videos.arumoon.ru/a/vapecig/video-channels

https://fedimovie.com/a/vapecig/video-channels

https://peertube.eus/a/vapecig/video-channels

https://tv.pirateradio.social/a/vapecig/video-channels

https://kumi.tube/a/vapecig/video-channels

https://vnhup.com/a/vapecig/video-channels

https://peertube.tweb.tv/a/vapeicgnew/video-channels

https://tv.suwerenni.org/a/vapecig/video-channels

https://peervideo.club/a/vapecig/video-channels

https://peertube.hosnet.fr/a/vapecig/video-channels

https://www.neptube.io/a/vapecig/video-channels

https://seka.pona.la/a/vapecig/video-channels

https://video.sadmin.io/a/vapecig/video-channels

https://pony.tube/a/vapecig/video-channels

https://the.jokertv.eu/a/vapecig/video-channels

https://peertube.su/a/vapecig/video-channels

https://peertube.ignifi.me/a/vapecig/video-channels

https://p2ptube.us/a/vapecig/video-channels

https://www.orion-hub.fr/a/vapecig/video-channels

https://peertube.zd.do/a/vapecig/video-channels

https://phijkchu.com/a/vapecig/video-channels

https://vhs.absturztau.be/a/vapecig/video-channels

https://varis.tv/a/vapecig/video-channels

https://cliptube.org/a/vapecig/video-channels

https://peertube.plasmatrap.com/a/vapecig/video-channels

https://peertube.ethibox.fr/a/vapecig/video-channels

https://video.antopie.org/a/vapecig/video-channels

https://peertube.gsugambit.com/a/vapecig/video-channels

https://3.600900.su/a/vapecig/video-channels

https://gade.o-k-i.net/a/vapecig/video-channels

https://tilvids.com/a/vapecignew/video-channels

https://videos.benjaminbrady.ie/accounts/vapecig/video-channels

https://peertube.wiesbaden.social/a/vapecig/video-channels

https://videos.skycademc.com/a/vapecig/video-channels

https://p.lu/a/vapecig/video-channels

https://yhwh.tube/a/vapecig/video-channels

https://peertube.ch/a/vapecig/video-channels

https://tube.nocturlab.fr/accounts/vapecig/video-channels

https://video.chalec.org/a/vapecig/video-channels

https://www.teachertube.com/user/channel/vapecig

https://video-cave-v2.de/a/vapecig/video-channels

https://peertube.redpill-insight.com/a/vapecig/video-channels

https://videos.shmalls.pw/a/vapecig/video-channels

https://tube.taker.fr/accounts/vapecig/about

https://www.mastersindia.co/q/profile/vapecig/

https://www.mycast.io/profiles/150955/username/ecigatornew

https://www.padelforum.org/members/vapecig.14616/#about

https://www.vwvortex.com/members/ecigatornew.3929812/#about

https://www.mdxers.org/members/ecigatornew.220878/#about

https://www.kiaownersclub.co.uk/members/vapecig.68908/#about

https://www.hondacivicforum.co.uk/members/ecigatornew.176825/#about

https://www.fordforums.com/members/ecigatornew.219928/#about

https://www.jeeprenegadeforum.com/members/ecigatornew.114594/#about

https://forums.fuwanovel.net/profile/32969-vape-factory/?tab=field_core_pfield_19

https://maptia.com/vape

https://fairygodboss.com/users/profile/j8j5VCtKFa/ecigator-new

https://os.mbed.com/users/vapecig/

https://p.mobile9.com/vapecig/

https://befonts.com/author/vapecig

https://forum.codeigniter.com/member.php?action=profile&uid=63282

https://hire.careerbliss.com/company/ecigator/

https://theprepared.com/members/7zd1MaRd8x/

https://video.barkoczy.social/a/vapecig/video-channels

https://bittube.video/a/vapecig/video-channels

https://www.collegethink.com/uemsconnect/members/vapecig/profile/

https://www.agileventures.org/users/vape-wholesale

https://www.demilked.com/author/vapecig/

https://www.theoutbound.com/vape-wholesale

https://pravatami.bg/profile/vapecig/

https://www.designspiration.com/ecigator/saves/

https://socialsocial.social/pin/ecigator-tikup-6000-puffs-disposable-vape-pod-cup/

https://socialsocial.social/user/vapecig/

https://onedio.com/profil/vapecig

https://lichess.org/@/vapecig

https://www.kiva.org/lender/vape1862

https://pinshape.com/users/2427866-vapecig

http://info.uniden.com/twiki/bin/view/Main/VapeFactory

https://www.anime-planet.com/users/vapecig

https://www.anibookmark.com/user/vapecig.html

https://community.flexispy.com/index.php?/profile/34541-vapecig/&tab=field_core_pfield_13

https://greensock.com/profile/128451-vapecig/?tab=field_core_pfield_11

https://forum-auto.caradisiac.com/profile/356827-vapecig/?tab=field_core_pfield_1

https://forums.bohemia.net/profile/1187784-vapecig/?tab=field_core_pfield_141

https://forums.auran.com/trainz/member.php?1218115-vapecig

https://www.pubpub.org/user/vape-factory

https://forums.bestbuy.com/t5/user/viewprofilepage/user-id/2737022

https://www.muvizu.com/Profile/vapecig/Latest/

https://pxhere.com/en/photographer/3877682

https://allauthor.com/profile/vapecig/

https://profile.sampo.ru/ecigator

https://www.ourboox.com/i-am/vape-factory/