Skip to main content
SearchLoginLogin or Signup

Reviews of: "Laboratory Validation and Clinical Implementation of Human Monkeypox Saliva-Based Tests"

Reviewers: P Fernandes (Instituto Politecnico de Viana do Castelo) | πŸ“™πŸ“™ ◻️◻️◻️ β€’ E Michailidou & P Athanasios (Aristotle University) | πŸ“’πŸ“’πŸ“’β—»οΈβ—»οΈ

Published onSep 07, 2022
Reviews of: "Laboratory Validation and Clinical Implementation of Human Monkeypox Saliva-Based Tests"
key-enterThis Pub is a Review of
Laboratory Validation and Clinical Implementation of Human Monkeypox Saliva-Based Tests
Description

AbstractBackgroundImproved diagnostic capabilities and accessibility are essential for controlling the outbreak of Human Monkeypox.MethodsWe describe a saliva-based polymerase chain reaction (PCR) assay for Human Monkeypox, in vitro test performance, and clinical implementation of that assay at three testing sites in Los Angeles. Finally, using pre-specified search terms, we conducted a systematic rapid review of PubMed and Web of Science online databases of studies reporting the performance of oral pharyngeal or saliva-based tests for Human Monkeypox.ResultsLaboratory evaluation of the assay showed in silico inclusivity of 100% for 97 strains of Human Monkeypox, with an analytic sensitivity of 250 copies/mL, and 100% agreement compared to known positive and negative specimens. Clinical testing identified 22 cases of Human Monkeypox among 132 individuals (16.7%). Of those 22 cases, 16 (72.7%) reported symptoms, 4 (18.2%) without a rash at the time of testing. Our systematic rapid review identified 6 studies; 23 patients had tests performed on oropharyngeal specimens 100% agreed with the PCR test result of a lesion swab.ConclusionSaliva-based PCR tests are potential tools for outbreak control, and further evaluation of the performance of such tests is warranted.

To read the original manuscript, click the link above.

Summary of Reviews: This preprint describes a saliva-based polymerase chain reaction (PCR) assay for Human Monkeypox Virus and assesses its in vitro test performance and clinical performance. Reviewers found this study to be potentially informative to not informative, highlighting several gaps in the study design and reporting of results that render it difficult to draw meaningful conclusions from the preprint without major clarification or further research.

Reviewer 1 (Paulo F…) | πŸ“™πŸ“™ ◻️◻️◻️

Co-Reviewer 2 (Evangelia M…) | πŸ“’πŸ“’πŸ“’ ◻️◻️

Co- Reviewer 2 (Poulopoulos A…) | πŸ“’πŸ“’πŸ“’ ◻️◻️

RR:C19 Strength of Evidence Scale Key

πŸ“• ◻️◻️◻️◻️ = Misleading

πŸ“™πŸ“™ ◻️◻️◻️ = Not Informative

πŸ“’πŸ“’πŸ“’ ◻️◻️ = Potentially Informative

πŸ“—πŸ“—πŸ“—πŸ“—β—»οΈ = Reliable

πŸ“˜πŸ“˜πŸ“˜πŸ“˜πŸ“˜ = Strong

To read the reviews, click the links below.Β 


Comments
2
?
sasafg hsea:

Cricket can cricket results be a game of ups and downs, with matches often lasting several hours or even days. Players learn to cope with setbacks, bounce back from failures, and persevere through challenging situations. This resilience can be valuable in facing obstacles in other aspects of life.

Vape Factory:

http://sgm.controlminero.gob.ec/web/vapecig/home?p_p_id=33

http://sgm.controlminero.gob.ec/web/vapecig/home/-/blogs/what-should-i-choose-for-my-first-electronic-cigarette

http://sgm.controlminero.gob.ec/web/vapecig/home/-/blogs/is-it-true-that-smoking-and-e-cigarettes-are-bad-for-arthritis-?

http://sgm.controlminero.gob.ec/web/vapecig/home/-/blogs/health-hazards-posed-by-new-tobacco

https://mapman.gabipd.org/web/anastassia/home/-/message_boards/message/222984

https://mapman.gabipd.org/web/anastassia/home/-/message_boards/message/222992

https://mapman.gabipd.org/web/anastassia/home/-/message_boards/message/222999

https://mapman.gabipd.org/web/anastassia/home/-/message_boards/message/223006

https://www.contratacionbogota.gov.co/vi/web/vapefactory/home

https://www.contratacionbogota.gov.co/vi/web/vapefactory/home/-/blogs/advantages-and-disadvantages-of-the-pod-type-vape

https://www.contratacionbogota.gov.co/vi/web/vapefactory/home/-/blogs/recommended-pod-type-vape

https://www.contratacionbogota.gov.co/vi/web/vapefactory/home/-/blogs/what-vapde-devices-do-you-recommend

https://www.contratacionbogota.gov.co/vi/web/vapefactory/home/-/blogs/5-disposable-vapes-recommended

https://caxman.boc-group.eu/web/vapecig/home

https://caxman.boc-group.eu/web/vapecig/home/-/blogs/what-brands-are-recommended-for-vape-beginners-

https://caxman.boc-group.eu/web/vapecig/home/-/blogs/vape-brands-recommended-for-vape-beginners

https://www.laonsw.net/web/vapecig

https://www.laonsw.net/web/vapecig/home/-/blogs/types-and-characteristics-of-cigarettes

https://www.kooymantracking.com/web/vapecig

https://www.kooymantracking.com/web/vapecig/home/-/blogs/which-electronic-cigarette-vape-is-better

https://www.kooymantracking.com/web/vapecig/home/-/blogs/vape-brand-recommendations

https://s100.ust21.kr/web/vapecig

https://s100.ust21.kr/web/vapecig/home/-/blogs/vape-brand-recommendations

https://s100.ust21.kr/web/vapecig/home/-/blogs/which-electronic-cigarette-vape-is-better

https://sensornet.lepida.it/web/vapecig/home

https://sensornet.lepida.it/web/vapecig/home/-/blogs/which-electronic-vape-for-cigarettes-is-superior

https://sensornet.lepida.it/web/vapecig/home/-/blogs/vape-brand-recommendations

https://www.syracuseultimate.org/web/vapecig

https://www.syracuseultimate.org/web/vapecig/home/-/blogs/which-electronic-vape-for-cigarettes-is-superior

https://www.syracuseultimate.org/web/vapecig/home/-/blogs/what-brands-are-recommended-for-vape-beginners

https://www.ehu.eus/eu/web/miguelsanchezmazaskatedra/foro/-/message_boards/message/41903570

https://www.ehu.eus/eu/web/miguelsanchezmazaskatedra/foro/-/message_boards/message/41904114

https://business.go.tz/web/rashid.ndimbo/~/86020/home/-/message_boards/message/27805080

https://business.go.tz/web/rashid.ndimbo/~/86020/home/-/message_boards/message/27805152

https://business.go.tz/web/rashid.ndimbo/~/86020/home/-/message_boards/message/27805192

https://business.go.tz/web/rashid.ndimbo/~/86020/home/-/message_boards/category/27805166

http://www.scientix.eu/es_ES/web/scientix-cop-4/topic-1/-/message_boards/message/1310427

http://www.scientix.eu/es_ES/web/scientix-cop-4/topic-1/-/message_boards/message/1310435

http://1to1.eun.org/web/acer/work-area/-/wiki/Main/What+is+the+recommended+vape+brands/pop_up

http://1to1.eun.org/web/acer/work-area/-/wiki/Main/Explosive+e-cigarettes+and+vapes/maximized

https://liferay.enaikoon.de/web/vapecig

https://liferay.enaikoon.de/web/vapecig/home/-/wiki/Main/How+to+travel+with+a+disposable+vape

http://www.tsv-unterthuerheim.de/web/vapecig/home

http://www.tsv-unterthuerheim.de/web/vapecig/home/-/blogs/how-long-does-a-disposable-vape-pen-la-1

http://www.tsv-unterthuerheim.de/web/vapecig/home/-/blogs/how-can-i-charge-a-disposable-vape-pen

http://opr.provincia.caserta.it/web/vapecig

http://opr.provincia.caserta.it/web/vapecig/home/-/blogs/what-disposable-vape-lasts-the-longest

http://opr.provincia.caserta.it/web/vapecig/home/-/blogs/vaping-vs-smoking-is-vaping-bad-for-you-

https://nmmc.imtrac.in/web/vapecig/home

https://nmmc.imtrac.in/web/vapecig/home/-/blogs/what-is-the-best-disposable-vape

https://nmmc.imtrac.in/web/vapecig/home/-/blogs/what-makes-prefilled-disposable-vape-different

https://topgradeapp.com/zh/lesson/the-rise-of-disposable-vapes-a-convenient-solution-or-an-environmental-disaster

https://topgradeapp.com/zh/lesson/exploring-the-pros-and-cons-of-disposable-vapes

https://topgradeapp.com/zh/lesson/understanding-the-health-risks-of-disposable-vapes

https://boosty.to/vapelife/posts/0dc771b0-5994-49ab-a78a-5a149bcf4f5a

https://boosty.to/vapelife/posts/9916b93f-3857-42f4-9c36-4a359b273abb

https://boosty.to/vapelife

https://k12.instructure.com/eportfolios/295556

https://k12.instructure.com/courses/930642/pages/pod-type-vape-is-a-hot-item

https://k12.instructure.com/courses/930642/pages/where-can-i-purchase-pod-type-vape

https://uws-ce.instructure.com/eportfolios/14146

https://hhi.instructure.com/courses/29901/pages/how-do-you-use-a-pod-vape

https://hhi.instructure.com/courses/29901/pages/what-vapde-devices-do-you-recommend

https://collab.phys.unsw.edu.au:4432/bin/view/Main/Vapefactory

https://collab.phys.unsw.edu.au:4432/bin/view/Main/Disposablevape

https://collab.phys.unsw.edu.au:4432/bin/view/Main/Podvape

https://collab.phys.unsw.edu.au:4432/bin/view/Main/Puffs

https://collab.phys.unsw.edu.au:4432/bin/view/Main/SkinCare