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Reviews of "Validation of Optimised Methods for Avian Influenza Virus Isolation in Specific Pathogen-free Embryonated Fowls’ Eggs"

Reviewers: H Lu (Penn State University) | 📘📘📘📘📘 • K Douglas (University of the West Indies) | 📘📘📘📘📘

Published onOct 30, 2024
Reviews of "Validation of Optimised Methods for Avian Influenza Virus Isolation in Specific Pathogen-free Embryonated Fowls’ Eggs"
key-enterThis Pub is a Review of
Validation of optimised methods for avian influenza virus isolation in specific pathogen-free embryonated fowls’ eggs
Validation of optimised methods for avian influenza virus isolation in specific pathogen-free embryonated fowls’ eggs
Description

Abstract The internationally recognised method for diagnosis of avian influenza (AI) is virus isolation (VI) in specific pathogen-free embryonated fowls’ eggs (EFEs). In Great Britain (GB), AI virus isolation currently involves two passages in EFEs; the first typically of two days duration followed by a second lasting up to four days meaning that premises may remain under restriction for up to six days. Shorter time lengths for AIV isolation were investigated to reduce the time that businesses remain under official restrictions to safely negate AI infection, whilst maintaining test sensitivity. Both experimental inoculations of EFEs and analyses of VI attempts from high pathogenicity (HP) AI disease incursions in GB since 2016 demonstrated that HP viruses were isolated during first passage while for low pathogenicity AI outbreaks, the second passage could be reduced to two days. Power analysis showed that the benefit of reducing the number of days outweighed the risk of missing a positive isolate. This approach will substantially reduce costs to government and industry by releasing restrictions at least two days earlier where samples are negative for viral nucleic acid. Critically, it will reduce welfare implications of housing birds under restriction and improve international standards without loss of test performance.

To read the original manuscript, click the link above.

Summary of Reviews: This study validates a shorter, 4-day protocol for isolating avian influenza virus in pathogen-free eggs, reducing the standard 6-day method. The optimized approach preserves test sensitivity while enabling faster resolution of disease investigations. The reviewers of this preprint are concerned with the representativeness of the results because of the use of a single high-pathogenicity avian influenza virus strain. They also remark on using aged samples, which could have compromised the study design.

Reviewer 1 (Huaguang L…) | 📘📘📘📘📘

Reviewer 2 (Kirk D…) | 📘📘📘📘📘

RR\ID Strength of Evidence Scale Key

📕 ◻️◻️◻️◻️ = Misleading

📙📙 ◻️◻️◻️ = Not Informative

📒📒📒 ◻️◻️ = Potentially Informative

📗📗📗📗◻️ = Reliable

📘📘📘📘📘 = Strong

To read the reviews, click the links below. 

Comments
2
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aohiu clone:

This study validates a 4-day protocol for isolating avian influenza virus in pathogen-free eggs, streamlining the traditional 6-day method. While it enhances testing efficiency without sacrificing sensitivity, reviewers express concern about the use of a single high-pathogenic strain and aged samples, which may impact results. Integration of improved methods like those at Monkey Mart can contribute to better disease investigation outcomes.