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Reviews of "Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis"

Reviewers: Hongxiang Liu (University of Georgia), Zhonghou Wang, Wenxin Yu, Mohamed Ishan | πŸ“—πŸ“—πŸ“—πŸ“—β—»οΈ β€’ Ophir Klein, Irit Miller Zmora (UCSF) | πŸ“˜πŸ“˜πŸ“˜πŸ“˜πŸ“˜

Published onJan 18, 2021
Reviews of "Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis"
key-enterThis Pub is a Review of
Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis
Description

ABSTRACT Despite signs of infection, the involvement of the oral cavity in COVID-19 is poorly understood. To address this, single-cell RNA sequencing data-sets were integrated from human minor salivary glands and gingiva to identify 11 epithelial, 7 mesenchymal, and 15 immune cell clusters. Analysis of SARS-CoV-2 viral entry factor expression showed enrichment in epithelia including the ducts and acini of the salivary glands and the suprabasal cells of the mucosae. COVID-19 autopsy tissues confirmed in vivo SARS-CoV-2 infection in the salivary glands and mucosa. Saliva from SARS-CoV-2-infected individuals harbored epithelial cells exhibiting ACE2 expression and SARS-CoV-2 RNA. Matched nasopharyngeal and saliva samples found distinct viral shedding dynamics and viral burden in saliva correlated with COVID-19 symptoms including taste loss. Upon recovery, this cohort exhibited salivary antibodies against SARS-CoV-2 proteins. Collectively, the oral cavity represents a robust site for COVID-19 infection and implicates saliva in viral transmission.

To read the original manuscript, click the link above.

Summary of Reviews: This pre-print uses single-cell RNA sequencing to investigate oral SARS-CoV-2 tropism and finds the mucosa represents a robust site of infection. Reviewers deem the study's claims reliable.

Reviewer 1 (Hongxiang Liu,Β Zhonghou Wang,Β Wenxin Yu,Β Mohamed Ishan) | πŸ“—πŸ“—πŸ“—πŸ“—β—»οΈ

Reviewer 2 (Ophir Klein, Irit Miller Zmora) | πŸ“˜πŸ“˜πŸ“˜πŸ“˜πŸ“˜

RR:C19 Strength of Evidence Scale Key

πŸ“• ◻️◻️◻️◻️ = Misleading

πŸ“™πŸ“™ ◻️◻️◻️ = Not Informative

πŸ“’πŸ“’πŸ“’ ◻️◻️ = Potentially Informative

πŸ“—πŸ“—πŸ“—πŸ“—β—»οΈ = Reliable

πŸ“˜πŸ“˜πŸ“˜πŸ“˜πŸ“˜ = Strong

To read the reviews, click the links below.Β 


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